La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Maximising the power of discrimination is important in microsatellite-based paternity analysis in songbirds
Auteurs :	Micaela Poetsch, Auteur ; Eva Baehnisch, Auteur ; Fritz Ludescher, Auteur ; Philip Dammann, Auteur
Type de document :	Article
Dans :	Journal of Ornithology (153(3) JUL 2012)
Article en page(s) :	873-880
Langues:	Anglais
Résumé :	Even though a considerable number of genetic markers are available for paternity analysis in birds, significant difficulties can arise in the assessment and interpretation of data. Paternity analyses using genetic markers are based on comparing similarities and dissimilarities (mismatches) between candidate fathers and their putative offspring. Complications arise when only a few mismatches are being detected, because any non-shared allele might well be caused by a mutation rather than being indicative of a true exclusion. To deal with such problems, it is essential to apply marker panels with sufficient discriminative power. Studying urban populations of Parus major, we report new combinations of microsatellite markers with extremely high sensitivity. We show that even marker panels with numerically satisfying discrimination power and exclusion probability (> 0.99999999 and > 0.999, respectively) do not always protect from insecurities. We also show that further maximisation of panel sensitivity by adding more markers can be helpful to resolve the majority of such ambiguous cases.

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