La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Development of 12 microsatellite loci for the endangered Pale-headed Brushfinch (Atlapetes pallidiceps) and their cross-amplification in two co-occurring Brushfinches
Auteurs :	Stefanie A. Hartmann, Auteur ; H. Martin Schaefer, Auteur ; Gernot Segelbacher, Auteur
Type de document :	Article
Dans :	Journal of Ornithology (155(3) JUL 2014)
Article en page(s) :	835-839
Langues:	Anglais
Résumé :	Using 454 pyro-sequencing, we established a microsatellite dataset of 12 polymorphic markers for the endangered Pale-headed Brushfinch (Atlapetes pallidiceps). Within 24 individuals, mean number of alleles ranged from 2 to 13, observed heterozygosity from 0.17 to 1.0, expected heterozygosity from 0.16 to 0.91, and polymorphic information content from 0.15 to 0.88. Additionally, we tested our marker set for cross-amplification in two related brushfinch species. Whereas all 12 markers amplified and were polymorphic in A. latinuchus, only 5 of the

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