La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	A comparison of flow cytometry and the polymerase chain reaction as sexing techniques for the Wood Thrush
Auteurs :	R. M. Underwood, Auteur ; R. J. Crockett, Auteur ; R. R. Roth, Auteur ; C. L. Keeler, Auteur ; M. S. Parcells, Auteur
Type de document :	Article
Dans :	Journal of Field Ornithology (73(3) SUM 2002)
Article en page(s) :	239-245
Langues:	Anglais
Résumé :	Gender identification is important in demographic studies of bird populations but is difficult with monomorphic bird species. Flow cytometry, a method which estimates differences in DNA content in erythrocyte nuclei, is one potential sexing method. In another sexing method, differences between the autosomal and sex-linked (W) copies of the chromo-helicase-DNA binding (CHD) gene can be determined using the polymerase chain reaction (PCR). Frozen blood samples collected during the breeding season from known-sex adults and juvenile birds were used to test the applicability of flow cytometry and PCR as sexing methods for the Wood Thrush (Hylocichla mustelina). With flow cytometry 86% of

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