La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Microsatellite typing of sperm trapped in the perivitelline layers of avian eggs: a cautionary note
Auteurs :	J. G. Martinez, Auteur ; T. Burke, Auteur
Type de document :	Article
Dans :	Journal of Avian Biology (34(1) MAR 2003)
Article en page(s) :	20-24
Langues:	Anglais
Résumé :	Recently Carter et al. (2000) have shown that it is possible to amplify microsatellite markers from the DNA of spermatozoa trapped in the perivitelline layers of avian eggs. This makes it possible to establish whether sperm reaches the ovum and to identify the males that contribute sperm competing to fertilise an egg. In this article we quantity the genotyping error rate associated with the biased amplification of differently sized alleles. We conclude that multiple marker loci should be used and that those markers where the competing males' alleles differ by more than ten base pairs should be avoided.

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