La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Assessment of CHD-specific primers for gender determination in Red-billed Oxpeckers Buphagus erythrorhynchus
Auteurs :	Desire L. Dalton, Auteur ; Antoinette Kotze, Auteur ; Mark Howitt, Auteur
Type de document :	Article
Dans :	Ostrich (81(3) NOV 2010)
Article en page(s) :	251-257
Langues:	Anglais
Résumé :	Red-billed Oxpeckers Buphagus erythrorhynchus are morphologically similar and do not display distinctive phenotypic difference between males and females. The development of DNA-based gender determination techniques constituted a breakthrough in reliable sex determination in birds. Two DNA-based methods of gender determination were evaluated to determine the preferred method for the Red-billed Oxpeckers. DNA-based gender determination of the Red-billed Oxpeckers was conducted so that specific sexes could be relocated to new release sites within South Africa. The two primer sets used were 2550F/2718R and P2/P8. When comparing the results of the two primer sets, it was determined that 17% (n = 25) of individuals that were identified as having one sex by the 2550F/2718R primer set changed their DNA gender determination when the P2/P8 primer set was used. Based on molecular evidence and the pathology results for three recorded mortalities at the National Zoological Gardens of South Africa, it was determined that the P2/P8 primer set would be preferable to the 2550F/2718R primer set for DNA gender determination of Red-billed Oxpeckers.

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