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Résumé :
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The effects of acute and chronic exposure to tributyltin (TBT) were examined in bioassays using horseshoe crab (Limulus polyphemus) embryos and "trilobite" larvae. Larvae had >95% survival after 24-h exposure to nominal concentrations of 1-500 mu g I(-1) TBT. Survival was also high following 48-h and 72-h exposure to less than or equal to 100 mu g l(-1) TBT; >50% mortality was seen only after 48-h and 72-h exposure to 500 g l(-1) TBT. Estimated median lethal concentrations (LC(50)) were >1000 mu g l(-1), 742 mu g l(-1), and 594 mu g l(-1) for 24-h, 48-h, and 72-h exposure, respectively. Much higher toxicity (LC,, = 42 mu g l(-1)) was seen following chronic exposure of larvae to TBT. Acute exposure to TBT significantly increased the time required by larvae to molt into the first-tailed stage. LC,, for horseshoe crab embryos exposed to TBT were
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