| Titre : | Use of the marine prophage induction assay (MPIA) to detect environmental mutagens |
| Auteurs : | L. D. McDaniel, Auteur ; V. McGee, Auteur ; J. H. Paul, Auteur |
| Type de document : | Article |
| Dans : | Estuaries (28(4) AUG 2005) |
| Article en page(s) : | 634-642 |
| Résumé : | The prophage induction assay provides a biologically based carcinogen-screening tool for environmental samples grounded in the parallel mechanisms of carcinogenesis and prophage induction. We developed an assay using a previously characterized marine bacterial Pseudomonas aeruginosa isolate designated as P944S3 for the detection of potentially genotoxic contamination in marine and estuarine environments. To perform the assay, the lysogenic isolate was exposed to either a known genotoxic compound or an environmental sample of interest. The response was considered positive when a statistically significant amount of prophage induction occurred in comparison to negative controls. Initial development of the assay for environmental samples included testing under a range of salinities and optimizing the method for the processing of water column and sediment samples. The assay has been field-tested over 2 yr in the Rookery Bay National Estuarine Research Reserve, Florida. The Marine Prophage Induction Assay (MPIA) was performed concurrently with laboratory toxicological analysis. There was good correspondence between positive MPIA results and detection of potentially toxic compounds by laboratory analysis. Five positive laboratory detections of known toxic compounds in natural samples occurred in conjunction with positive MPIA results. Two laboratory detections of compounds that are not genotoxic were accompanied by a negative MPIA response. Eight of the sediment samples contained detectable levels of arsenic. Four of these samples demonstrated a positive MPIA response, which may be due to the oxidation state of the arsenic within the sediment. One detection of a known toxic compound by the analytical laboratory was not accompanied by a positive induction response. Nine positive induction responses occurred without concurrent laboratory detection. This was possibly due to the limited range of compounds included in the laboratory testing performed, although false positive assay results cannot be ruled out. |
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