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Résumé :
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Alkaline phosphatase (AP) was detected using ELF-97A (R) in silicon-starved Pseudo-nitzschia multiseries cells; thus, we tested two, alternative hypotheses: Pseudo-nitzschia multiseries has a high phosphate demand, showing signs of phosphate deficiency even when concentrations of orthophosphate are high, or silicate deficiency can stimulate the AP enzyme in this species. We also studied the effect of silicon deficiency on AP in three other common marine diatoms: Thalassiosira pseudonana, Nitzschia pusilla, and Nitschia closterium. Each of the species tested showed a different pattern of AP regulation.
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