La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Reliable and rapid discrimination of congeneric species by mtDNA SNP analysis by multiplex PCR: application on three Trachurus and two Mullus fish species as model cases
Auteurs :	Apostolos P. Apostolidis, Auteur ; Andreas Georgiadis, Auteur ; Nikoletta Karaiskou, Auteur ; Raphael Sandaltzopoulos, Auteur
Type de document :	Article
Dans :	Hydrobiologia (614(1) NOV 2008)
Article en page(s) :	401-404
Résumé :	We present a reliable, time-saving, and cost-effective multiplex PCR assay for discriminating congeneric species. Three fish species of the genus Trachurus and two of the genus Mullus served as model cases. Our multiplex PCR method interrogates species-specific diagnostic mitochondrial single nucleotide polymorphisms (mtSNPs). We selected two sets of mtSNPs that are organized in two multiplex systems for the Trachurus and the Mullus species, respectively. In both systems, all individuals tested could be safely assigned to one of the three Trachurus or two Mullus species. This novel SNP typing system offers a convenient and robust DNA profiling system suitable for large-scale identification of commercial fish species, for species with cryptic larvae or during juvenile stages, as well as for wildlife forensics.

Lundi , mardi et jeudi de 9h à 12h et de 13h à 17h00 : ouverture au public ...