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Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Different methods and storage duration affect measurements of epilithic extracellular enzyme activities in lotic biofilms
Auteurs :	Nathan J. Smucker, Auteur ; Jared L. DeForest, Auteur ; Morgan L. Vis, Auteur
Type de document :	Article
Dans :	Hydrobiologia (636(1) DEC 2009)
Article en page(s) :	153-162
Résumé :	Extracellular enzyme activity (EEA) is becoming increasingly common for measuring biofilm function in streams. Different methods for enzyme assays may yield results that cannot be compared among studies, and duration of sample storage may also affect EEAs, leading to erroneous conclusions. We compared two frequently used methods for measuring phosphatase (PHOS), leucine aminopeptidase (LAMP), beta-glucosidase (GLU), and beta-xylosidase (XYLO) by conducting assays with intact and disrupted epilithic biofilms grown on tiles in three streams. Storage duration effects on EEA were documented with intact and disrupted biofilms kept in the dark at 4A degrees C for 3 and 5 days. Intact biofilms had significantly less EEA than disrupted biofilms for all enzymes (P

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