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Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Recent advances in pigment analysis as applied to picophytoplankton (2005)
Auteurs :	M. Zapata, Auteur
Type de document :	Article
Dans :	Vie et Milieu - Life and Environment (55(3-4) décembre 2005)
Article en page(s) :	233-248
Langues:	Anglais
Résumé :	Advances in chromatographic (HPLC) and characterization techniques (LC-MS, NMR) applied to the study of the chloroplast pigments have changed our current understanding of picophytoplankton pigment diversity. Improved HPLC protocols, with special selectivity toward very similar pigments, have allowed the separation of mono (MV) and divinyl (DV) forms of chls a, b, and c, and the separation of close structurally related carotenoids. The application of pigment analysis to the study of cultured prokaryotic and eukaryotic picophytoplankton has rendered a reappraisal of its pigment composition. The pigment types obtained for photosynthetic organisms are useful to infer the diversity, abundance, and spatial and temporal distribution of picophytoplankton in field samples. The study of plastid pigments at distinct taxonomical levels, from Kingdom to ecotype, throws light on picophytoplankton taxonomy, ecophysiology, and phylogeny. To summarise the pigment diversity found in picoeukaryotes, pigment-based chloroplast types are defined and discussed in the context of chemotaxonomy, secondary endosymbiosis and plastid evolution.
En ligne :	https://wwwphp.obs-banyuls.fr/Viemilieu/index.php/volume-55-2005/55-issue-3-4.html

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