La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	GAPDH as a control gene to estimate genome copy number in Great Tits, with cross-amplification in Blue Tits (2013)
Auteurs :	Els Atema, Auteur ; Kees van Oers, Auteur ; Simon Verhulst, Auteur
Type de document :	Article
Dans :	Ardea (101(1) Spring, 2013)
Article en page(s) :	49-54
Résumé :	Estimating the number of genome copies in a tissue sample can serve various purposes. For example, such an estimate serves as a scaling variable when measuring telomeres with quantitative PCR. We describe the primer development and evaluation for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene in the Great Tit Parus major, as a control gene to estimate genome copy number. We demonstrate specific amplification with negligible variation in 48 Great Tits and cross-amplification in 53 Blue Tits Cyanistes caeruleus. We conclude this primer set to be reliable for amplification of GAPDH as a reference gene for quantitative PCR analysis in Great and Blue Tits.

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