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Institut de recherche pour la conservation des zones humides méditerranéennes

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Titre :	Properties of bacteriocin like substances produced by Rhizobium sp ORN83 and ORN24 strains previously isolated in saline soils of Algeria
Auteurs :	Kacem Mourad, Auteur ; Kazouz Hafida, Auteur
Type de document :	Article
Dans :	Ecologia Mediterranea (35 2009)
Article en page(s) :	91-98
Résumé :	Seven Rhizobium sp. strains previously isolated in the saline region of Algeria were screened for their antagonistic activity by a well diffusion method. Among these, seven strains caused an inhibition zone of 6 to 20 mm in diameter using indicator strain Sinorhizobium sp. ORN16. Cell free supernatants from Rhizobium sp. ORN24 (FII24) and ORN83 (FII83) were subject to different treatment. Enzymes (alpha-chymotrypsin, trypsin or proteinase K) had no effect on the activity of FII83, whereas the inhibitory activity of FII24 was completely abolished by the same enzymes, reflecting the proteinaceous nature of the inhibitory agent produced by Rhizobium sp. ORN24 strain. Additionally, complete inactivation of SII83 was observed when it was exposed to 100 degrees C for 20 min, while FII24 showed resistance to heat, retaining all activity after exposure to 100 degrees C for 60 min. Both FII83 and FII24 are bactericidal, and retained their antibacterial activity within the range of pH 4 to 7 and maintained full stability after storage for 60 days at 4 degrees C, 0 degrees C and at - 20 degrees C, while no activity was detected after storage for 80 to 120 days at 37 degrees C. Tween 80, Tween 20, SDS or Triton X-100 seems to have a marked effect on FII24; in contrast the activity of FII83 was not reduced when treated with these agents. The antibacterial substance present in FII24 appears to have the properties commonly associated with "true" bacteriocins and showed inhibition against some strains of Rhizobium sp., Sinorhizobium sp., Propionibacterium, Lactobacillus and Erwinia chrysanthemi. Pseudomonas savastanoi CFBP 2074 and Pseudomonas savastanoi 13519a, two phytopathogenic bacteria that causes olive nodes (olive knot) or tuberculosis were also inhibited by FII24. This suggests good potential for the use of the Rhizobium sp. ORN24 strain or it's FII234 as a biocontrol agent to prevent plant diseases caused by Pseudomonas savastanoi or Erwinia chrysanthemi species.

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