La Tour du Valat

Institut de recherche pour la conservation des zones humides méditerranéennes

Titre :	Molecular phylogeny of Heritiera Aiton (Sterculiaceae), a tree mangrove: variations in RAPD markers and nuclear DNA content
Auteurs :	Anath Bandhu Das, Auteur ; Arup Kumar Mukherjee, Auteur ; Premananda Das, Auteur
Type de document :	Article
Dans :	Botanical Journal of the Linnean Society (136(2) 2001-06-01)
Article en page(s) :	221-229
Résumé :	Random amplified polymorphic DNA (RAPD) markers are used to estimate interspecific variation among mangrove and non-mangrove Heritiera fomes, H. littoralis and H. macrophylla. All the species have 2n = 38 chromosomes, with minute structural changes distinguishing the karyotype of each species. Significant variation of 4C DNA content occurs at the interspecific level. Interspecific polymorphism ranged from 14.09% between H. fomes and H. littoralis to 52.73% between H. fomes and H. macrophylla. H. macrophylla showed wide polymorphism in the RAPD marker with H. littoralis (51.23%) and H. fomes (52.73%). Two distinct RAPD products obtained from OPA-10 (1000 bp) and OPD-15 (900 bp) found characteristic molecular markers in H. macrophylla, a species from a non-mangrove habitat. H. macrophylla was more distantly related to H. fomes [genetic distance (1-F) = 0.305] than to H. littoralis [genetic distance (1-F) = 0.273]. H. littoralis was of a closer affinity to H. fomes [genetic distance (1-F) = 0.218] than to H. macrophylla.
En ligne :	https://doi.org/10.1111/j.1095-8339.2001.tb00567.x

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